| 產(chǎn)品編號 | bs-0180R |
| 英文名稱 | DOPA Decarboxylase Rabbit pAb |
| 中文名稱 | 多巴胺脫羧酶抗體 |
| 別 名 | AADC; DDC; Aromatic L Amino Acid Decarboxylase; DDC protein; DOPA decarboxylase; aromatic-L-amino-acid decarboxylase isoform 1; DDC_HUMAN. |
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Specific References (1) | bs-0180R has been referenced in 1 publications.
[IF=1.29] Hiramoto, Keiichi, Yurika Yamate, and Shosuke Kawanishi. "Detection of Dopa‐positive cells in mouse ovaries in response to ocular exposure to ultraviolet B rays." Photodermatology, Photoimmunology & Photomedicine (2014). IHC-F ; Mouse.
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| 研究領域 | 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞外基質 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DDC: 201-300/480 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
DOPA decarboxylase is an enzyme implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Following the hydroxylation of tyrosine to form L dihydroxyphenylalanine (LDOPA), catalyzed by tyrosine hydroxylase, DDC decarboxylates LDOPA to form dopamine. This neurotransmitter is found in different areas of the brain and is particularly abundant in basal ganglia. Dopamine is also produced by DDC in the sympathetic nervous system and is the precursor of the catecholaminergic hormones, noradrenaline and adrenaline in the adrenal medulla. In the nervous system, tryptophan hydroxylase produces 5 OH tryptophan, which is decarboxylated by DDC, giving rise to serotonin. DDC is a homodimeric, pyridoxal phosphate dependent enzyme. Function: Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Subunit: Homodimer. DISEASE: Defects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive. Similarity: Belongs to the group II decarboxylase family. SWISS: P20711 Gene ID: 1644 Database links: Entrez Gene: 1644 Human Entrez Gene: 13195 Mouse Omim: 107930 Human SwissProt: P20711 Human SwissProt: O88533 Mouse Unigene: 359698 Human Unigene: 12906 Mouse Unigene: 11064 Rat 該抗體主要用于：神經(jīng)細胞退行性改變-老年滯呆的研究 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Cerebrum (Mouse) Tissue Lysate at 40 ug
Lane 2: adrenal gland (Mouse) TissueLysate at 40 ug
Lane 3: Cerebrum (Rat) Tissue Lysate at 40 ug
Lane 4: adrenal gland (Rat) Tissue Lysate at 40 ug
Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 6: HepG2 (Human) Cell Lysate at 30 ug
Lane 7: Jurkat (Human) Cell Lysate at 30 ug
Lane 8: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-DOPA Decarboxylase (bs-0180R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 50 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
