| 產(chǎn)品編號 | bs-4026R |
| 英文名稱 | ALDOB Rabbit pAb |
| 中文名稱 | 醛縮酶2抗體 |
| 別 名 | ALDB; ALDO B; ALDO2; ALDOB; ALDOB_HUMAN; Aldolase 2; Aldolase B; Aldolase B fructose bisphosphate; Aldolase2; AldolaseB; EC 4.1.2.13; Fructose bisphosphate aldolase B; Fructose-bisphosphate aldolase B; Liver type aldolase; Liver-type aldolase; MS1077. |
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Specific References (1) | bs-4026R has been referenced in 1 publications.
[IF=3.345] Xu et al. Morphological and proteomic analyses reveal that unsaturated guluronate oligosaccharide modulates multiple functional pathways in murine macrophage RAW264.7 cells. (2015) Mar.Drug. 13:1798-818 WB ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ALDOB: 261-364/364 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ALDOB is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Defects in ALDOB cause hereditary fructose intolerance. Subunit: Homotetramer. DISEASE: Hereditary fructose intolerance (HFI) [MIM:229600]: Autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the class I fructose-bisphosphate aldolase family. SWISS: P05062 Gene ID: 229 Database links: Entrez Gene: 229 Human Entrez Gene: 230163 Mouse Omim: 612724 Human SwissProt: P05062 Human SwissProt: Q91Y97 Mouse Unigene: 530274 Human Unigene: 482116 Mouse Unigene: 98207 Rat 醛縮酶（ALD）與機(jī)體能量代謝密切相關(guān)。醛縮酶含量比較豐富，分布也叫廣泛，醛縮酶B是一種與糖酵解有關(guān)的酶類，在人類和哺乳動物組織中存在3種醛縮酶同工酶，即A(肌型)，B(肝型)和C（腦型）。其中B型基因定位于q13-q32，屬胞漿酶。研究表明，ALDOB在肝細(xì)胞癌發(fā)展過程中呈現(xiàn)出一定規(guī)律的變化，與原發(fā)性肝癌有關(guān)。ALDOB蛋白主要定位于胞漿。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human SH-SY5Y cell lysates
Primary: Anti-ALDOB (bs-4026R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-ALDOB (bs-4026R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ALDOB) polyclonal Antibody, Unconjugated (bs-4026R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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