| 產品編號 | bs-11367R |
| 英文名稱 | SYNPR/Synaptoporin Rabbit pAb |
| 中文名稱 | 突觸囊泡蛋白抗體 |
| 別 名 | SPO; Synaptophysin 2; Synaptoporin; Synpr; SYNPR_HUMAN; DKFZp686G0883; MGC26651. |
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Specific References (2) | bs-11367R has been referenced in 2 publications.
[IF=7.26] Zhang, Chi, et al. "The potential use of H102 peptide-loaded dual-functional nanoparticles in the treatment of Alzheimer’s disease." Journal of Controlled Release (2014). WB ; Mouse.
[IF=1.177] Zhenting Huang . et al. Anti-Cognitive Decline by Yinxing-Mihuan-Oral-Liquid via Activating CREB/BDNF Signaling and Inhibiting Neuroinflammatory Process. 2021 Jan 27 IF,IHC ; Rat.
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| 研究領域 | 細胞生物 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rat (predicted: Human,Mouse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 29 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Synaptoporin: 31-130/265 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Synaptic vesicle recycling involves numerous proteins that contribute to the formation and trafficking of the SNARE complexes throughout the cell. Synaptoporin, also designated synaptophysin 2, is an integral membrane protein of small synaptic vesicles that belongs to the synaptophysin/synaptobrevin family. Synaptoporin is highly homologus to Synaptophysin 1 and both Synaptoporin and synaptophysin 1 contain four transmembrane domains and a short cytoplasmic tail. The Synaptoporin protein also contains one MARVEL domain, a membrane-associating domain found in lipid-associating proteins, and displays calcium-binding activity which may be localized to its cytoplasmic tail. Syntaphilin, synaptophysin and Synaptoporin regulate the formation of the vesicles by competing with components of the SNARE complexes to respectively inhibit either the assembly or the secretion of the synaptic vesicles. Function: Intrinsic membrane protein of small synaptic vesicles. Probable vesicular channel protein. Subcellular Location: Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Similarity: Belongs to the synaptophysin/synaptobrevin family. Contains 1 MARVEL domain. SWISS: Q8TBG9 Gene ID: 132204 Database links: Entrez Gene: 132204 Human Entrez Gene: 72003 Mouse SwissProt: Q8TBG9 Human SwissProt: Q8BGN8 Mouse Unigene: 648668 Human Unigene: 317515 Mouse Unigene: 211986 Rat |
| 產品圖片 |
Sample:
Bulbus olfactorius (Rat) Lysate at 40 ug
Primary: Anti-Synaptoporin (bs-11367R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYNPR) Polyclonal Antibody, Unconjugated (bs-11367R) at 1:800 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-SYNPR/Synaptoporin antibody(bs-11367R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
