| 產品編號 | bs-15385R |
| 英文名稱 | GPR161 Rabbit pAb |
| 中文名稱 | G蛋白偶聯受體GPR161蛋白抗體 |
| 別 名 | FLJ33952; G-protein coupled receptor 161; G-protein coupled receptor RE2; GP161; GPR161; RE2; GP161_HUMAN. |
| 研究領域 | 細胞生物 免疫學 信號轉導 G蛋白偶聯受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 59 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR161: 121-220/529 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G-protein coupled receptors translate extracellular signals into intracellular signals (G-protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR161 (G protein-coupled receptor 161), also known as RE2, is a 529 amino acid protein that belongs to the G-protein coupled receptor family. Localized to the cell membrane, GPR161 is a multi-pass membrane protein that functions as an orphan receptor, relaying extracellular signals to the intracellular environment. Two isoforms of GPR161 exist due to alternative splicing events. Function: Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. Its ligand is unknown (By similarity). Subcellular Location: Cell projection, cilium membrane; Multi-pass membrane protein (By similarity). Cell membrane; Multi-pass membrane protein (By similarity). Note=Mainly localizes to primary cilium in a TULP3 and IFT-A complex-dependent manner. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes and is apparently not degraded (By similarity). Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q8N6U8 Gene ID: 23432 Database links: Entrez Gene: 23432 Human Entrez Gene: 240888 Mouse SwissProt: Q8N6U8 Human SwissProt: B2RPY5 Mouse Unigene: 271809 Human Unigene: 329929 Mouse |
| 產品圖片 |
Sample:
Hela (Human)cell Lysate at 30 ug
Primary: Anti- GPR161 (bs-15385R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 65 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
