| 產(chǎn)品編號 | bs-14428R |
| 英文名稱 | DRP2 Rabbit pAb |
| 中文名稱 | 肌營養(yǎng)不良蛋白相關(guān)蛋白2抗體 |
| 別 名 | DRP-2; Drp2; DRP2_HUMAN; Dystrophin-related protein 2. |
| 研究領域 | 細胞生物 發(fā)育生物學 神經(jīng)生物學 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 110 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DRP2: 801-900/957 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Members of the dystrophin family of proteins perform a critical role in the maintenance of membrane-associated complexes at points of intercellular contact in vertebrate cells. The protein encoded by this gene is predicted to resemble certain short C-terminal isoforms of dystrophin and dystrophin-related protein 1 (DRP1 or utrophin). DRP2 is expressed principally in the brain and spinal cord. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010] Function: Possibly involved in membrane-cytoskeleton interactions of the central nervous system. Subcellular Location: Cytoplasm > cytoskeleton. Tissue Specificity: Brain and spinal cord; very low levels in eye, ovary, epididymis and testis; not in cardiac and skeletal muscle. Similarity: Contains 2 spectrin repeats. Contains 1 WW domain. Contains 1 ZZ-type zinc finger. SWISS: Q13474 Gene ID: 1821 Database links: Entrez Gene: 1821 Human Entrez Gene: 13497 Mouse Omim: 300052 Human SwissProt: Q13474 Human SwissProt: Q05AA6 Mouse Unigene: 159291 Human Unigene: 121662 Mouse Unigene: 162341 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Cerebrum (Mouse) Tissue Lysate at 40 ug
Lane 2: Cerebrum (Rat) Tissue Lysate at 40 ug
Lane 3: BV-2 (Mouse) Cell Lysate at 30 ug
Lane 4: Huvec (Human) Cell Lysate at 30 ug
Lane 5: Molt-4 (Human) Cell Lysate at 30 ug
Lane 6: SH-SY5Y (Human) Cell Lysate at 30 ug
Primary: Anti-DRP2 (bs-14428R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110 kD
Observed band size: 120 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DRP2) Polyclonal Antibody, Unconjugated (bs-14428R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
