| 產(chǎn)品編號 | bs-16107R |
| 英文名稱 | FLJ14213 Rabbit pAb |
| 中文名稱 | Protor2蛋白抗體 |
| 別 名 | FLJ14213 protein; Hypothetical protein FLJ14213; Hypothetical protein LOC79899; Proline-rich protein 5-like; Protein observed with Rictor-2; Protor 2; Protor-2; PROTOR2; PRR5L; PRR5L_HUMAN. |
| 研究領域 | 細胞生物 免疫學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FLJ14213: 231-330/368 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
mTOR is a large protein kinase that is important in cell growth and functions as the mammalian target of Rapamycin, an immunosuppressant that blocks vessel restenosis and also has potential anticancer applications. Rapamycin-insensitive companion of mTOR, also designated Rictor, forms a complex (designated mTORC2) with mTOR that directly phosphorylates Akt/PKB on Ser473 and plays a key role in growth signaling pathways. Protor-2, also known as PROTOR2 or FLJ14213, is a 368 amino acid protein that is thought to interact with the mTORC2 complex and, via this interaction, may regulate organization of the Actin cytoskeleton. Three isoforms of Protor-2 are expressed due to alternative splicing events. Function: May be part of the TORC2 complex which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. Subunit: Interacts with the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Interacts with RFFL. Post-translational modifications: Ubiquitinated. Ubiquitination by RFFL promotes proteasomal degradation of PRR5L thereby modifying the substrate-specific activity of the mTORC2 complex. Ubiquitination by RFFL is stimulated by LPA/lysophosphatidic acid. Similarity: Belongs to the PROTOR family. SWISS: Q6MZQ0 Gene ID: 79899 Database links: Entrez Gene: 79899 Human Entrez Gene: 72446 Mouse Omim: 611728 Human SwissProt: Q6MZQ0 Human SwissProt: A2AVJ5 Mouse Unigene: 19987 Human Unigene: 24138 Mouse Unigene: 19060 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
