| 產(chǎn)品編號 | bs-48014P |
| 英文名稱 | Recombinant human M-CSF protein (Active, HEK293) |
| 中文名稱 | 重組人M-CSF (活性的, Tag free) |
| 別 名 | M-CSF; M CSF; rhM-CSF; Macrophage Colony Stimulating Factors; Macrophage Colony Stimulating Factor 1; Colony stimulating factor 1 (macrophage); Colony stimulating factor 1; Colony stimulating factor macrophage specific; CSF 1; CSF1; CSF-1; Csfm; Lanimosti 人M-CSF重組蛋白; 重組人M-CSF; 人重組M-CSF; 重組人M-CSF; 重組人巨噬細胞集落刺激因子; 人巨噬細胞集落刺激因子; 巨噬細胞克隆刺激因子; 刺激因子; |
| 理論分子量 | kDa |
| 檢測分子量 | 35-40 |
| 性 狀 | Lyophilized |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | Human |
| 序 列 | 33-190/554 |
| 純 度 | >95% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內(nèi)毒素 | <0.5 EU/μg as determined by LAL. |
| 表達系統(tǒng) | HEK293 cell |
| 活性 | Yes |
| 標簽 | Tag free |
| 緩 沖 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存條件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
M-CSF (Macrophage Colony stimulating Factor) is a hematopoietic stem cell factor and produced by various cell types including lymphocytes, fibroblasts, endothelial cells, myoblasts, monocytes, and osteoblasts. It is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Recombinant human M-CSF is a 35-40 kDa homodimeric and glycosylated. This recombinant human M-CSF is produced by human cells.(Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line). Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| 產(chǎn)品圖片 |
The purity of the protein is greater than 95% as determined by reduced and non reduced SDS-PAGE.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
