| 產(chǎn)品編號 | bs-41337R |
| 英文名稱 | AMPK alpha-1 Rabbit pAb |
| 中文名稱 | 腺苷單磷酸活化蛋白激酶α1抗體 |
| 別 名 | AMPK alpha-1; 5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; AAPK1; acetyl CoA carboxylase kinase; AI194361; AI450832; AL024255; A |
| 研究領域 | 細胞生物 神經(jīng)生物學 激酶和磷酸酶 Alzheimer's |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 64 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human AMPK alpha-1 protein : 1-207/559 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] Gene ID: 5562 Database links:
Entrez Gene: 5562 Human
Entrez Gene: 105787 Mouse Omim: 602739 Human SwissProt: Q13131 Human SwissProt: Q5EG47 Mouse Unigene: 43322 Human Unigene: 207004 Mouse Unigene: 87789 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HUVEC cell lysates
Lane 2: Human SH-SY5Y cell lysates
Primary: Anti-AMPK alpha-1 (bs-41337R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Human AMPK alpha-1 protein, His Tag (Cat. bs-41337P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human AMPK alpha-1 Antibody, the EC50 is 133.7 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human AMPK alpha-1 protein, His Tag (Cat. bs-41337P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human AMPK alpha-1 Antibody, the EC50 is 13.01 ng/mL.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
