| 產(chǎn)品編號(hào) | bsm-60189M |
| 英文名稱 | Butyryl-Histone H3 (Lys18) Mouse mAb |
| 中文名稱 | |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | B10D8 |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | Antigen affinity purification |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 組蛋白經(jīng)過各種酶催化的修飾，包括乙酰化，甲基化，磷酸化，泛素化等。賴氨酸丁?；切掳l(fā)現(xiàn)的一種控制染色體結(jié)構(gòu)和基因轉(zhuǎn)錄的可逆修飾。賴氨酸丁?；趶娜湎x到人的真核細(xì)胞中高度保守。組蛋白賴氨酸丁?；?dú)特的結(jié)構(gòu)和基因組定位表明，它在機(jī)制和功能上不同于組蛋白賴氨酸乙?；＞唧w地說，在人類體細(xì)胞和兔雄性生殖細(xì)胞基因組中，組蛋白丁?；瘶?biāo)志著活性啟動(dòng)子或潛在增強(qiáng)子。組蛋白H3在Lys18位點(diǎn)的丁?；赡茉诒碛^遺傳調(diào)控中發(fā)揮重要作用，包括染色質(zhì)重塑和DNA轉(zhuǎn)錄調(diào)控。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) C2C12，(+) C2C12+Sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 17 kDa
Observed MW: 17 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa，(+) HeLa+Sodium butyrate (30mM, 4hr)+Trichostatin A (500ng/ml, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 17 kDa
Observed MW: 17 kDa
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