| 產(chǎn)品編號 | bs-17611R-Bio |
| 英文名稱 | Rabbit Anti-CCDC49/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的卷曲螺旋結(jié)構(gòu)域蛋白49抗體 |
| 別 名 | Coiled-coil domain-containing protein 49; CWC25_HUMAN; Pre-mRNA-splicing factor CWC25 homolog. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 50kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CCDC49 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Spliceosomes are large ribonucleoproteins that remove introns from nuclear pre-mRNA in a two-step reaction. CCDC49 is a 425 amino acid protein that is involved in the catalytic steps of splicing in Saccharomyces cerevisiae. CCDC49 associates with a component of the Prp19-associated complex, CEF1, which is involved in spliceosome activation. It is likely that CCDC49 facilitates juxtaposition of the 5’ splice site and branch point during the final step in the first catalytic reaction. There are two isoforms of CCDC49 that are produced as a result of alternative splicing events. Similarity: Belongs to the CWC25 family. Database links: Entrez Gene: 91050 Human Entrez Gene: 100503884 Mouse SwissProt: Q6ZUS6 Human Unigene: 106432 Human Unigene: 332379 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
